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(a) Effect of serum deprivation on TRPC6 cell surface abundance and the regulation by soluble Klotho. HEK cells expressing TRPC6 were cultured in serum deprived or serum containing media for 24h and further incubated with soluble Klotho (200pM) for 2h before subjecting to biotinylation assay. The abundance of tubulin in lysates served as a loading control.
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